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Antibody Isotypes

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Inhibition of renalase expression and signaling has antitumor activity in
The sections were blocked in 3% hydrogen peroxide for 30 min and 2.5% normal horse serum in PBS/0.1% Tween20 for 1 hr followed by incubation with primary antibody and isotype control IgG overnight at 4 °C.
Longer telomeres in chronic, moderate, unconjugated hyperbilirubinaemia
Briefly, heparinized whole blood was treated with brefeldin A solution for 4 h, to block cytokine excretion. Red blood cells were lysed (Cell Lysis Solution 1:10, BD) and cells were stained with CD14 surface antibody (Biolegend).
Earlier infantile immune maturation is related to higher DTP vaccine responses
The most apparent multivariate pattern was that higher anti-DTP antibody titers at 18 months of age were associated with lower infantile total counts of T and B cells in the blood.
Near Infrared Fluorescence (NIRF) Molecular Imaging of Oxidized LDL with an
(A,B) Direct staining of en face preparations of aortic arches from Ldlr−/− mice, using (A) LO1-750, or (B) isotype control IgG3-750. Staining with the VivoTag-S 750-MAL labeled antibodies is red and nuclei counterstained with SYTO-24 are green.
Histone acetyltransferease p300 modulates TIM4 expression in dendritic cells
After washing with PBS, the cells were incubated with fluorochrome-labeled antibodies of interest for 30 min on ice. A portion of the cells was stained with isotype IgG using as a negative staining control. The cells were analyzed with a flow cytometer ...

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