Sequencing Maxam Gilbert Sequencing
- Digestion of Nucleic Acids Starts in the Stomach
- At first, the digestion of various DNA and RNA sequences by pepsin was investigated. As shown in Fig. 2a, digestion by pepsin was observed for DNA extracted from salmon sperm, bacteriophage λ, plasmid pET-28a, and M13mp18 phage.
- Comparative next-generation sequencing of adeno-associated virus inverted
- Previously, complete ITR sequences could only be obtained using the Maxam-Gilbert sequencing approach (13, 14) or Sanger sequencing with the addition of betaine or 7-deaza-dGTP (15). Amplification and sequencing of AAV ITRs is required for studying ...
- Future of Next-Gen Sequencing
- With the advent of both Sanger and Maxam and Gilbert DNA sequencing technologies in the late 1970s, just the mere ability to sequence individual DNAs of interest was a great leap forward.
- Watson: 'DNA was my only gold rush'
- Cold Spring Harbor, New York (CNN) -- At 85, James Watson still plays tennis every week. His hips and knees haven't given out yet, he says, and he likes grass courts.
- The future of DNA sequencing: an interview with Barrett Bready, CEO of Nabsys
- The first methods for sequencing DNA were developed in the late 1970s. Frederick Sanger developed rapid DNA sequencing methods and published a method for "DNA sequencing with chain-terminating inhibitors,” while Walter Gilbert and Allan Maxam ...
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