- Long noncoding RNA MALAT1 promotes hepatic steatosis and insulin resistance by
- HepG2 cells were transfected pcDNA-MALAT1 and si-MALAT1 for 48 h, then nuclear (n-) SREBP-1c expression (A) intracellular levels of triglycerides (B) and cholesterol (C) were measured. **P < 0.01, compared to pcDNA.
- c.A2456C-substitution in Pck1 changes the enzyme kinetic and functional
- In addition, and especially in tissues where no gluconeogenesis occurs, it plays a fundamental role in glyceroneogenesis, providing glycerol-3-phosphate as a precursor for fatty acid esterification in triglyceride synthesis.
- Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells
- Induction of apoptosis was confirmed by annexin V-fluorescein/propidium iodide (FITC/PI) co-staining (Becton Dickinson, Franklin Lakes, NJ, USA) using a flow cytometer. At 20h after irradiation 58% of PBMCs were annexin V/PI positive (supplementary Fig.
- Deletion of Cyclophilin D Impairs β-Oxidation and Promotes Glucose Metabolism
- (F,G) Pancreas sections from WT or CypD KO mice were stained with an antibody to insulin by immunohistochemistry (F), and labeled areas were quantified and multiplied by relative intensity (G).
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